NGS sequencing-based oncology panels

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Frontage offers cost-effective examination of oncogenes from tumor tissue, biofluids, or genomic or cfDNA samples using targeted sequencing of oncopanels developed by industry leading manufacturers such as Illumina and Swift Biosciences. Only panels with expert–defined content are utilized in order to identify variants and fusion events with established relevance to cancer genetics. Extraction services are optimized for multiple samples types including biofluids to facilitate examination of circulating genetic variants collected with minimally invasive strategies.

96 well insert - Oncopanels

Examining Genetic Variants with Liquid Biopsy and Tumor Samples

Frontage Laboratories provide ultra-deep sequencing of cancer genes as well as cancer-related genes included in oncopanels. The targeted genes in these oncopanels are among the most studied genes in cancer research.

Our approach allows direct analysis of potentially pathogenic genetic variants residing in known oncogenes. In addition, we can isolate cell-free DNA (cfDNA) from patient blood samples, and genomic DNA from tissue biopsies for ultra-deep sequencing of targeted oncogenes. The focused nature of oncopanels can reduce costs and increase the speed of genomic studies because only a limited set of genes are interrogated. Additional benefits include advanced detection of low-frequency variants with deeper sequencing options and production of more manageable datasets than traditional whole genome sequencing studies.

Oncogene Analysis Service

A wide choice of oncopanels are available for selection at Frontage for oncogene analysis service (Table 1). Many of the oncopanels accommodate low input DNA (10 ng) and are therefore amenable to cfDNA oncogene analysis. While some oncopanels include sets of genes involved in various tumor types, others are more specific and contain genes involved in specific cancer types e.g. colorectal cancer and lung cancer. The diversity as well as the specificity of the oncopanels available enable selection of the best oncopanel that satisfies your research interests.

Table I. Oncopanels available for analysis using ORB’s oncogene analysis service.

Panel Genes Cancer Type Manufacturer FFPE cfDNA oncogene analysis
  TruSight Cancer Sequencing 94   Pan-Cancer Illumina
  TruSight Tumor 15 15   Solid Tumor Illumina Yes
  TruSight Myeloid Sequencing Up tp 54   Hematological Illumina
  AmpliSeq – Cancer Hotspot Panel v2 48   Pan-Cancer Illumina Yes
  TruSight Tumor 170 170   Solid Tumor Illumina Yes
  AmpliSeq – Custom DNA Panel Up to 12,000 amplicons   Pan-Cancer Illumina Yes
  TruSight Oncology 500 523   Pan-Cancer Illumina Yes Yes
  Accel-Amplicon 56G Oncology Panel v2 56   Multiple Swift Biosciences Yes Yes
  Accel-Amplicon Plus 57G Pan-Cancer Profiling Panel 57   Pan-Cancer Swift Biosciences Yes
  Accel-Amplicon BRCA1 and BRCA2 2   Breast and Ovarian Cancer Swift Biosciences Yes Yes
  Accel-Amplicon BRCA1, BRCA2, and PALB2 3   Breast and Ovarian Cancer Swift Biosciences Yes Yes
  Accel-Amplicon Comprehensive TP53 1   Multiple Swift Biosciences Yes Yes
  Accel-Amplicon EGFR Pathway 4   Multiple Swift Biosciences Yes Yes
  Accel-Amplicon™ Plus Colorectal (CRC) Cancer Panel 16   Colorectal Cancer Swift Biosciences Yes Yes
  Accel-Amplicon™ Plus Lung Cancer Panel 17   Lung Cancer Swift Biosciences Yes Yes
  Accel-Amplicon™ Plus Myeloid Panel 23   Hematological Swift Biosciences Yes Yes
  xGen®Acute Myeloid Leukemia Cancer Panel >260   Acute Myeloid Leukemia IDT
  xGen®Pan-Cancer Panel 127   Pan-Cancer IDT
  Human Breast Cancer Panel 93   Breast Cancer Qiagen Yes
  Human Colorectal Cancer Panel 71   Colorectal Cancer Qiagen Yes
  Human Lung Cancer Panel 72   Lung Cancer Qiagen Yes
  Human Myeloid Neoplasms Panel 141   Hematological Qiagen Yes
  Human Actionable Solid Tumor Panel 23   Solid Tumor Qiagen Yes
  Human BRCA1 and BRCA2 Panel 2   Breast and Ovarian Cancer Qiagen Yes
  Human BRCA1 and BRCA2 Plus Panel 6   Breast and Ovarian Cancer Qiagen Yes
  Human Comprehensive Cancer Panel 160   Multiple Qiagen Yes
  SeqCap EZ Comprehensive Cancer Design 578   Multiple Roche
  SeqCap EZ Human Oncology 981   Multiple Roche
  CleanPlex® OncoZoom Panel 65   Multiple Paragon Genomics Yes Yes

 Common Oncology Areas Utilizing Oncopanels

  • Breast cancer
  • Melanoma
  • Colorectal cancer
  • Pancreatic cancer
  • Ovarian cancer
  • Prostate cancer
  • Non-small cell lung cancer
  • Renal cell carcinoma

Sample Types and Sample Submission Instructions

  • Tumor biopsies
  • Genomic DNA (gDNA)
  • Cell-free DNA (cfDNA)
Sample Type Minimum Input Requirement Sample Submission Checklist
Tumor tissue 10 mg Tissue
Genomic or cell-free DNA 10 ng gDNA / cfDNA
Biofluid 4 ml Biofluid